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. 2017 Apr 1;26(4):693–702. doi: 10.3727/096368916X694184

Figure 3.

Figure 3.

Regulator of cell cycle (rgcc) inhibition rescues PC12 cells from cell death induced by nerve growth factor (NGF) withdrawal. (A) Bar graph shows relative levels of cell survival as measured by the LIVE/DEAD assay [mean ± standard deviation (SD); arbitrary units] for PC12 cultures maintained on NGF, deprived of NGF for 48 h, deprived of NGF for 48 h in the presence of rgcc siRNA, or deprived of NGF for 48 h in the presence of scrambled control siRNA. (B) Bar graph shows relative levels of cyclin b1 (ccnb1) transcript levels as measured by quantitative polymerase chain reaction (qPCR) (mean ± SD; arbitrary units) for PC12 cultures maintained on NGF, deprived of NGF for 48 h, deprived of NGF for 48 h in the presence of rgcc siRNA, or deprived of NGF for 48 h in the presence of scrambled control siRNA. *p < 0.05; *p < 0.01 versus NGF, via one-way analysis of variance (ANOVA) with Bonferroni post hoc comparisons.