Choline acetyltransferase (ChAT) and anti-glutamic acid decarboxylase (GAD-6) expression in the grafted neurons. (A-F) Grafted cholinergic neurons identified with immunohistochemistry (IHC) labeling for STEM121 and ChAT expression in jaundice (jj; A-C) and nonjaundice (Nj; D-F) brains. Images show STEM121, Ku80, and merged. (G-I) GAD-6-labeled neuron within grafted tissue in an Nj brain. (G) STEM121; (H) GAD-6; (I) merge. There were limited numbers of cholinergic or GABAergic neurons presented in the graft. g, graft; arrow, cells; st, striatum. Scale bars: 50 μm (A-C, G-I), 20 μm (D-F).