Table 1.
Custom‐designed ovine specific probes and primers used for real‐time quantitative RT‐PCR determinations of target mRNA levels in ovine fetal tissues
| Gene | Primer/probe | Nucleotide sequence | Amplicon size |
|---|---|---|---|
| VEGF164 | Forward | GGCGAGGCAGCTTGAGTTAA | 75 |
| Reverse | CACCGCCTCGGCTTGTC | ||
| Probe | 6FAMCGAACGTACTTGCAGATGMGBNFQ | ||
| VEGF164b | Forward | AACACAGACTCGCGTTGCAA | 63 |
| Reverse | GGTGAGACGTCTGCAAGTACGTT | ||
| Probe | 6FAMCGAGGCAGCTTGAGMGBNFQ | ||
| sFlt‐1 | Forward | TGCCGAGCTAGGAACATATACACA | 103 |
| Reverse | AGATCCGAGAGAAAACAGCCTTT | ||
| Probe | 6FAMAAGAAATCCTCCAGAAGAAMGBNFQ | ||
| Caveolin‐1 | Forward | TGTGATTGCAGAACCAGAAGGA | 61 |
| Reverse | GGTGAAGCTGGCCTTCCA | ||
| Probe | 6FAMCACACAGTTTCGATGGCMGBNFQ | ||
| Flotillin‐1 | Forward | CACAGAGGGACTACGAGCTGAA | 58 |
| Reverse | CGGCGCGTGTTGACTTC | ||
| Probe | 6FAMAAGGCCGCATACGACMGBNFQ | ||
| Dynamin‐2 | Forward | CGGCTGAGAGGAAGTTTTTCC | 61 |
| Reverse | CCCATGCGGTCCGCTAT | ||
| Probe | 6FAMACCCAGCCTACCGGCMGBNFQ | ||
| Tubulin‐α | Forward | ACGTGGTTCCCAAAGATGTCA | 66 |
| Reverse | GCTGCGCTTGGTCTTGATG | ||
| Probe | 6FAMTGCTGCCATTGCCMGBNFQ | ||
| HIf‐1α | Forward | CCATGCCCCAGATTCAAGAT | 59 |
| Reverse | ACTTTGTCTGGTGCTTCCATCA | ||
| Probe | 6FAMAGCCAGCTAGTCCTTMGBNFQ | ||
| PBEF | Forward | TGATCCCAACAAAAGGTCCAA | 63 |
| Reverse | AAATTTCCTGCTGGTGTCCTATG | ||
| Probe | 6FAMAAGGGCCGATTATCMGBNFQ |