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Immunohistochemical staining of osteogenesis‐ and osteoclastogenesis‐related proteins in human cancellous bone of old and young individuals. Sample size for aged individuals was n = 11 (RUNX2), 8 (ALP), 11 (OCN) and 11 (RANKL); for young individuals n = 5 (RUNX2), 6 (ALP), 5 (OCN) and 5 (RANKL). Additionally, the isotype control for each type of antibody is shown. Data are presented as mean ± S.E.M.; Student's t‐test. (*P < 0.05). Scale bar: 20 μm.
