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. 2017 May 30;21(11):3113–3116. doi: 10.1111/jcmm.13220

Figure 2.

Figure 2

Effect of PTEN and NOX4 on replicative‐induced MMP1 expression in HDFs. Replicative‐aged HDFs (55 PD) were cultured in standard medium and infected with Ad/LacZ or Ad/PTEN. The levels of NOX4 and MMP1 expression after PTEN gene transfer or transfected with NOX4‐targeting siRNAs were analysed by real‐time PCR (A or C) and Western blotting (B or D). (E) In transfected replicative‐aged HDF cells with NOX4‐targeting siRNAs, ROS was measured by flow cytometry using DCFH‐DA. Each value represents the mean ± S.E.M. of three independent experiments. # P < 0.01 vs. Ad/lacZ or control siRNA.