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. 2017 Jun 9;21(11):2926–2936. doi: 10.1111/jcmm.13204

Figure 5.

Figure 5

Effects of cimifugin on CLDND1, CLDN‐1 and occludin in HaCaT cells. (A), CLDND1, CLDN‐1 and occludin expressions were analysed by Western blot in HaCaT cells (n = 3). (B–D), CLDND1, CLDN‐1 and occludin expressions relative to β‐actin were quantified by ChemiScope analysis. (E, F), immunofluorescence quantification of CLDN‐1 and occludin expressions (n = 3, magnification: ×200; mean + SD, n = 3, # P < 0.05, ## P < 0.01, versus control, *P < 0.05, **P < 0.01 versus TNF‐α). The data are representatives of three independent experiments.