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. 2017 May 24;21(11):2909–2925. doi: 10.1111/jcmm.13203

Figure 6.

Figure 6

FGF2 contributes to maintain mitochondrial KATP channel expression and functional integrity. (A) Expression of mitochondrial ATP‐dependent potassium (KATP) channel subunit Kir6.2 was determined by immunofluorescence staining 2 days after reperfusion. Kir6.2 (in green) was widely distributed in renal tubular epithelial cells and was more abundant than VDAC (in red) in sham‐operated kidney, but Kir6.2 expression declined dramatically in I/R animals, which was largely reversed by FGF2 treatment. The effect of FGF2 in reversing the decrease of Kir6.2 expression is counteracted by 5‐HD co‐treatment. Results are representative of four animals from each group. (B) Western blot analysis of Kir6.2 protein expression. VDAC was used as an internal control. FGF2 treatment sustained Kir6.2 expression, but this effect was reversed by 5‐HD (mean ± S.E.; n = 4). **P < 0.01 versus sham group, #P < 0.05 versus I/R group, $P < 0.05 versus FGF2 + I/R group. (C) The optical density analysis of Kir6.2 in the kidney tissue sections.