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. 2017 May 24;21(11):2909–2925. doi: 10.1111/jcmm.13203

Table 1.

Primers used to amplify rat cDNAs

Gene GenBank Primer sequences
GAPDH NM_012675 5′‐ GACATGCCGCCTGGAGAAAC‐3′
5′‐AGCCCAGGATGCCCTTTAGT‐3′
IL‐1β NM_031512 5′‐TGCAGGCTTCGAGATGAAC‐3′
5′‐GGGATTTTGTCGTTGCTTGTC‐3′
IL‐6 NM_012589 5′‐AAGCCAGAGTCATTCAGAGC‐3′
5′‐GTCCTTAGCCACTCCTTCTG‐3′
KIM‐1 NM_173149 5′‐CTCTGTTGATAGTGATAGTGGTCTG‐3′
5′‐TGTGGGTCTTGTAGTTGTGG‐3′
TLR2 NM_198769 5′‐ATGAACACTAAGACATACCTGGAG‐3′
5′‐CAAGACAGAAACAGGGTGGAG‐3′
TLR4 NM_019178 5′‐CATGACATCCCTTATTCAACCAAG‐3′
5′‐GCCATGCCTTGTCTTCAATTG‐3′
TNFα NM_012675 5′‐CTTCTCATTCCTGCTCGTGG‐3′
5′‐TGATCTGAGTGTGAGGGTCTG‐3′

IL‐1β: interleukin‐1β; IL‐6: interleukin‐6; TLR2: Toll‐like receptor‐2; TLR‐4: Toll‐like receptor‐4; KIM1: Kidney Injury Molecule‐1; TNFα: tumour necrosis factor‐α; GAPDH: Glyceraldehyde 3‐phosphate dehydrogenase.