Figure 4.

Yap inhibitor suppresses UUO-induced kidney fibrosis. (A and B) Yap inhibitor suppresses UUO-induced myofibroblast accumulation. Mice with UUO were treated with DMSO or verteporfin (100 mg/kg body wt, i.p. every other day for four times). Markers of myofibroblasts, SMA-α and PDGFRα, and ECM proteins, fibronectin, and collagen I, were determined by western blot (A), with density analysis of (A) shown in (B) (*P<0.05 versus UUO group). (C and D) Immunohistochemistry analysis of myofibroblast marker SMA-α (C) was performed and the positive-staining area (D) was measured. (E and F) Kidney fibrosis was determined by trichrome and Sirius Red staining; the area of positive staining, the green color of trichrome staining, and the red color in Sirius red staining were summarized (E). (G and H) The tubule cell marker, E-cadherin, (G) and the endothelial cell marker, VE-cadherin (H), were determined by immunofluorescent staining in control and verteporfin-treated groups. n=5; *P<0.05.