Figure 6.

Yap/Taz KO inhibits the accumulation of proliferating cells in tubulointerstitium after UUO. (A) Yap/Taz KO had no effect on apoptotic cells in obstructed kidneys. Apoptosis was determined by TUNEL analysis; positive cells show green color in the nucleus. (B) Yap/Taz KO inhibits PCNA-positive cells in the tubulointerstitium of obstructed kidney. (C and D) Double immunostaining of PCNA and PDGFRα-positive cells in UUO kidney of WT and Yap/Taz KO mice. (E) The percentage of PCNA⁺/PDGFRα⁺ double-positive cells in total PDGFRα⁺ cells were summarized (n=5). (F) CD45 inflammatory cells are positively stained for PCNA in UUO kidney from YAP/Taz KO mice (n=5).