Figure 7.

Inducible KO of Yap/Taz reverses UUO-induced kidney fibrosis. (A–D) Release-UUO model was used to determine whether Yap/Taz KO in fibroblasts ameliorates fibrosis. UUO was released after 3 days; Yap/Taz KO mice were induced by adding tamoxifen for 5 days. Wild-type mice that received tamoxifen treatment as Yap/Taz KO mice were taken as control group. After 7 days, kidneys were collected and the expression of myofibroblast markers was detected by western blot (A) and immunohistochemistry (C). The corresponding density analyses are shown (B and D), respectively. (n=5; *P<0.05 compared with WT control group). (E and F) Fibrosis was detected by PAS, Sirius Red, and trichrome staining (E) and the positive areas were statistically analyzed (F). (n=5; *P<0.05).