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. 2017 Jul 24;28(11):3205–3217. doi: 10.1681/ASN.2016050508

Figure 2.

Figure 2.

The inflammatory cytokine IFN-γ regulates the transcriptional activity of HNF-1β. (A) Exposure to inflammatory cytokines downregulated the target genes of HNF-1β, such as KIF12 and CDH16, recapitulating what is observed after siRNA-mediated inhibition of HNF-1β. (B) Western blot analysis of HNF-1β revealed that IFN-γ downregulated the HNF-1β protein, recapitulating what is observed after siRNA-mediated inhibition of HNF-1β. (C) HK-2 cells were transfected with a Kif12 promoter-reporter plasmid, whose expression is dependent on HNF-1β.32 Measurement of the luciferase assay revealed that IFN-γ downregulates the transcriptional activity of HNF-1β, recapitulating what is observed after siRNA-mediated HNF-1β invalidation. *P<0.05; **P<0.01; ***P<0.001.