Figure 5.

PPARGC1A is a target gene of HNF-1β. (A) ChIP-qPCR analysis performed on mouse kidney chromatin using HNF-1β antibodies or control IgG. There is a significant enrichment on an HNF1-binding site identified in the Ppargc1a promoter compared with a region with no predicted HNF1 consensus sequence (NC, negative control). A known binding site in Pkhd1 promoter¹⁷ was used as a positive control. (B) The position of HNF1-binding sites and negative regions are indicated relative to the transcription start site TSS of the corresponding gene. Sequence conservation of HNF1-binding sites among different species is shown below the HNF1-binding sites. Results are expressed as percentage of immunoprecipitated DNA compared with input DNA and shown as mean±SEM. Statistical significance was determined by ANOVA analysis followed by Bonferroni post-test. ***P<0.001.