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. 2017 Jul 24;28(11):3205–3217. doi: 10.1681/ASN.2016050508

Figure 6.

Figure 6.

Inhibition of HNF-1β leads to mitochondrial disorders including a lower production of reactive oxygen species. (A) Relative RNA expression of a mitochondrial gene (ND-1 gene) and a nuclear gene (ACTNB) in HK-2 cells transfected with scrambled siRNA or an siRNA targeting HNF1B mRNA showed that the mitochondrial content was similar in both conditions. (B) Electron microscopy showed that inhibition of HNF-1β leads to mitochondrial swelling, partial cristae disappearance, and autophagolysosomes increase. Mitochondrion form ratio measurement was significantly reduced in Hnf1b−/− MCT cells compared with controls (n=282). (C) Real-time H2O2 measurement in the supernatant of HK-2 cells after IFN-γ exposure and HNF-1β inhibition. *P<0.05; **P<0.01; ***P<0.001.