Table I.
Gene | Orientation | Primer sequence |
---|---|---|
WT p21 | Forward | 5′-ATTGAGCTCTAATCCGCCCACAGGAAG-3′ |
Reverse | 5′-CTCAAGCTTACAAGTAAAGTCACTAAG-3′ | |
MU p21 | Forward | 5′-TGGGAACGACTGTCTTTCCTGGCACTAACGTT-3′ |
Reverse | 5′-AGACAGTCGTTCCCAGCCCCATATGAGCCCAC-3′ | |
WT p27 | Forward | F: 5′-CGCGAGCTCGAATTAAGAATATGTTTC-3′ |
Reverse | 5′-TTGACGCGTATGCAACCTTTTAAGCATAGC-3′ | |
MU p27 | Forward | 5′-CCGCTCGAGTGATCTGCCTCTAAAAGCGT-3′ |
Reverse | 5′-CGGGATCCATTCTTAACATTCAAAACTCCC-3′ |
WT, wild-type; MU, mutant.