Figure 3.

Effect of shRNAs on cell apoptosis in HeLa cells by flow cytometry analysis. The E1 quadrant was the cell debris, the E2 quadrant was the necrotic cell group, the E3 quadrant was the surviving cell group and the E4 quadrant was the apoptotic cell group. (A) Blank control; (B) shNC; (C) shRNA2; (D) shRNA3; (E) shRNA4; (F) shRNA5; and (G) shRNA6. The increase in the number of apoptotic cells was marked. shRNA, short hairpin RNA; shNC, negative control shRNA; FITC, fluorescein isothiocyanate.