Figure 1.

The effect of CA treatment on cell proliferation. (A) A375 cells were treated with CA for 24 h and cell proliferation was analyzed with an MTT assay (n=6). (B) A375 cells were treated with CA for 72 h and cell proliferation was analyzed by an MTT assay (n=6). (C) The number of viable A375 cells was determined with with trypan blue staining. The cells were treated with various concentrations of CA (0, 0.03, 0.1 and 0.3 µM), or bafilomycin A1 as a positive control, for 24 h (n=3). Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. control. CA, cleistanthin A; CON, control.