Morphologic appearance and morphometric assessments of regenerated nerves in each group.
Notes: (A–E) The characteristic toluidine blue photographs of regenerated axons in the autograft group, MG group, MG+MF group, MG+SCs group, and MG+SCs+MF group at 12 weeks postoperation, respectively. (F–O) The characteristic electron micrographs of regenerated axons in the middle part of the scaffold in the (F) autograft group, (G) MG group, (H) MG+MF group, (I) MG+SCs group, and (J) MG+SCs+MF group at 12 weeks postoperation. (K–O) The characteristic electron micrographs of myelin sheath in the middle part of the scaffold in the autograft group, MG group, MG+MF group, MG+SCs group, and MG+SCs+MF group at 12 weeks postoperation. (P) The cross-sectional area of regenerated nerves in the scaffold. (Q) Quantitative analysis of myelinated axons in the scaffold. (R) The diameter of myelinated axons in the scaffold. (S) The G-ratios in the scaffold. *p<0.05 compared to MG group; #p<0.05 compared to MG+MF group; &p<0.05 compared to MG+SCs group; all results are expressed as the mean ± standard error of mean.
Abbreviations: MG, the rats were bridged with the magnetic scaffold; MG+MF, the rats were bridged with the magnetic scaffold and under MF exposure after surgery; MG+SCs, the rats were bridged with the Schwann cells-loaded magnetic scaffold; MG+SCs+MF, the rats were bridged with the Schwann cells-loaded magnetic scaffold and under MF after surgery; MF, magnetic field; SCs, Schwann cells.