Table 2. Comparing epitope mapping result of HRH peptide with genuine VEGFR-binding site on ligand.

Receptor	Ligand	Complex	HRH peptide mapping results	PDB sum interface
VEGFR-1	VEGF-A	1FLT	K16, Q22, R23, H27, T31, L32, Q37, K48, R56, T71, T77, Q79, R82, Q89, H90, L97, Q98, H99, K101	F17, M18, Y21, Q22, Y25, K48, D63, G65, L66, M81, I83, H86, Q89, I91, E103, C104, R105, P106
VEGFR-1	VEGF-B	2XAC	T22, T25, L35, T36, L39, T42, A44, K45, Q46, L47, Q79, L81, R84, Q89, L90	S16, W17, Y21, T22, T25, Q27, V48, P62, D63, L66, Q79, L81, S88, Q89, L90, C103, P105
VEGFR-1	PlGF	1RV6	R35, A36, L37, R39, L63, R64, T66, T81, A82, Q106, H107, R109	F25, Q26, W29, G30, Y33, M54, S56, G70, D71, L74, N73, Q87, L89, I91, P97, Y99, C112, R113, P114
VEGFR-2	VEGF-A	3V2A	H27, T31, R56, L66, T71, L97, Q98, H99, K101, R105	P40, D41, I43, E44, K48, M81, I83, P85, H86, Q89
VEGFR-2	VEGF-C	2X1W	R127, K128, Q130, R134, A137, R161, L171, Q172, T176, H206, R210	T116, L119, D123, W126, R127, Q130, K142, T148, N149, F151, K153, N167, S168, E169, G170, F186, I188, V190, P191, L192, G195, P196, R210, K214, L215

Abbreviations: PlGF, placenta growth factor; VEGF, vascular endothelial growth factor; VEGFR, VEGF receptor.

Overlapping residues were in bold.