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. 2017 Mar 24;2:17005. doi: 10.1038/sigtrans.2017.5

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Construction of pACNR-JEV5ʹ and pACNR-JEV3ʹ plasmid. Seven fragments of JEV14-14-2 gene were obtained by PCR. The first three and the other four fragments were ligated and constructed into low-copy plasmid pACNR. The resulting plasmids were named pACNR-JEV5ʹ and pACNR-JEV3ʹ that contains JEV sequence from 1 to 3450 nt and from 3445 to 10 977 nt.