Table 2. Primers for constructing JEV-infectious DNA clone.

Primers	Sequences
F1	5′- TTGGCGCGCCATTTAGGTGACACTATAGAGAAGTTTATCTGTGTGAACTTCTT-3′, AscI sp6 promoter
R1	5′- CCTGGCGCCTGCACAAGCTATGACAAC-3′, KasI
F2	5′- TTGGCGCGCCAATGCAGGCGCCATGAAGTTGTCGAAT-3′, AscI, KasI
R3	5′- GGTCCGGACCAGTCTAGTGACAGATCTGACTC-3′, BspEI, BglII
F4	5′- TGCGGAGTCAGATCTGTCACT-3′, BglII
R4	5′- CATTTTCTGTCCGGAATCGTAG-3′, BspEI
F5	5′- CGCCCCTACGATTCCGGACAGA-3′, BspEI
R6	5′- GGAAAAGGATCCGTGGTTCCAGG-3′, BamHI
F7	5′- ACCACGGATCCTTTTCCTGACTC-3′, BamHI
R8	5′- CCGCTCGAGCGGTGCTCTAGAACGCGTGTATTCCGACGTGATCAGGTG-3′, XhoI, XbaI, MluI, BclI
F9	5′- CACCTGATCACGTCGGAATA-3′, BclI
R10	5′- CCGCTCGAGCCCAAAGCTTCAAACTCTAGAT-3′, XhoI, XbaI
F11	5′- GCTTGGAGCACGGTATCTAGAGTT -3′, XbaI
R11	5′- CCGCTCGAGAGATCCTGTGTTCTTCCTCACCAC-3′, XhoI

Serial nos	Mutations	SA14-14	SA14-14-2
1	E-107	Phenylalanine	Leucine
2	E-138	Lysine	Phenylalanine
3	E-176	Valine	Isoleucine
4	E-177	Alanine	Threonine
5	E-264	Methionine	Lysine
6	E-279	Valine	Alanine
7	E-315	Proline	Serine

Abbreviation: JEV, Japanese encephalitis virus. Underline entities are the restriction enzyme.