Table 3. Primers used to generate JEV mutations.

Primers	Sequences	Mutations
177-F	5′- CCAATGCTCCTTCGGTAACCCTCAAAC-3′	G to A
177-R	5′- GTTTGAGGGTTACCGAAGGAGCATTGG-3′	C to T
264-F	5′- GAAGGAGGCCTCCATCAGGCGTTG-3′	T to G
264-R	5′- CAACGCCTGATGGAGGCCTCCTTC-3′	A to C
279-F	5′ - GTACTCAAGCTCAGTGAAGTTAACATCAGG-3′	T to A
279-R	5′- CCTGATGTTAACTTCACTGAGCTTGAGTAC-3′	A to T
315-F	5′- GAAAAATCCGGCGGACACTGGTCAC-3′	T to C
315-R	5′- GTGACCAGTGTCCGCCGGATTTTTC-3′	A to G
F	5′- TTGGCGCGCCAATGCAGGCGCCATGAAGTTGTCGAAT-3′, AscI, KasI	A to C silent mutation
R	5′- GGTCCGGACCAGTCTAGTGACAGATCTGACTC-3′, BspEI, BglII

Abbreviation: JEV, Japanese encephalitis virus. Bold is the location of the mutation. The underline entities are the restriction enzyme sites.