Table 1. TP ODNs: sequences, molecular weight analysis and net charge at pH 7.4.
| TP ODN | Sequence |
Molecular weight
|
Net charge | |
|---|---|---|---|---|
| Calculated | Observed | |||
| 4K | 5′-TLysGTAALysACCLysATGATGTGCTGCTLysA-3′ | 7469.8 | 7468.3 | −17 |
| 6K | 5′-TLysGTAAALysCLysCLysALysTGATGTGCTGCTLysA-3′ | 7830.0 | 7828.9 | −15 |
| 4GR | 5′-TGRGTAAGRACCGRATGATGTGCTGCTGRA-3′ | 7806.0 | 7804.9 | −13 |
| 6GR | 5′-TGRGTAAAGRCGRCGRAGRTGATGTGCTGCTGRA-3′ | 8334.3 | 8333.8 | −9 |
| 2PA | 5′-TPepAGTAAACCATGATGTGCTGCTPepAA-3′ | 8130.3 | 8128.5 | −13 |
| 3PA | 5′ -TPepAGTAAACCAPepATGATGTGCTGCTPepAA-3′ | 8820.6 | 8820.4 | −9 |
| 2PG | 5′-TPepGGTAAACCATGATGTGCTGCTPepGA-3′ | 8252.3 | 8251.7 | −13 |
| 3PG | 5′ -TPepGGTAAACCAPepGTGATGTGCTGCTPepGA-3′ | 8988.8 | 8988.4 | −9 |
| 2PJ | 5′-TPepJGTAAACCATGATGTGCTGCTPepJA-3′ | 8526.4 | 8524.9 | −9 |
| 3PJ | 5′-TPepJGTAAACCAPepJTGATGTGCTGCTPepJA-3′ | 9414.9 | 9414.7 | −3 |
Abbreviations: ACHN, acetylated N terminus; GR, N3-Gly-Arg-NH2; NH2, amidated C terminus; PepA, AcHN-Lys(N3)-Lys-Lys-Arg-Gly-NH2; PepG, AcHN-Lys(N3)-Arg-Arg-Arg-Gly-NH2; PepJ, AcHN-Lys(N3)-Lys-Lys-His-His-His-NH2; TP ODN, triazolylphosphonate oligodeoxynucleotide.
The MALDI measurements were obtained in the negative ion mode. The number preceding the peptide/amino-acid abbreviation refers to the number of peptide triazoylphosphonate internucleotide linkages/TP ODN.