Table 2. TP ODN melting temperatures.
| ODN | Duplex | Modifications | T m | ΔT m | ΔT m per mod |
|---|---|---|---|---|---|
| ctrl | x′/x | 0 | 70.0 | — | — |
| 4K | 4K/x | 4 | 63.0 | −7.0 | −1.75 |
| 6K | 6K/x | 6 | 56.6 | −13.4 | −2.23 |
| 4GR | 4GR/x | 4 | 66.3 | −3.7 | −0.93 |
| 6GR | 6GR/x | 6 | 59.0 | −11.0 | −1.83 |
| 2PA | 2PA/x | 2 | 66.6 | −3.4 | −1.70 |
| 3PA | 3PA/x | 3 | 67.6 | −2.4 | −0.80 |
| 2PG | 2PG/x | 2 | 54.6 | −15.4 | −7.70 |
| 3PG | 3PG/x | 3 | 64.3 | −5.7 | −1.90 |
| 2PJ | 2PJ/x | 2 | 61.0 | −9.0 | −4.50 |
| 3PJ | 3PJ/x | 3 | 62.0 | −8.0 | −2.67 |
Abbreviations: mod, modification; TP ODN, triazolylphosphonate oligodeoxynucleotide; x, 5′-TAGCAGCACATCATGGTTTACA-3′; x′, 5′-TGTAAACCATGATGTGCTGCTA-3′.
The TP ODN sequences are defined in Table 1. All T ms represent an average of at least three experiments; ΔT m is the difference in T m when compared with T m of the unmodified duplex.