Table 3. Sequences of the TP ODNs studied in the luciferase assay.

Oligo	Sequence	Net charge
4K	5′-TLysGTAALysACCLysATGATGTGCTGCTLysA-3′	−17
6K	5′-TLysGTAAALysCLysCLysALysTGATGTGCTGCTLysA-3′	−15
2PB	5′-TPepBGTAAACCATGATGTGCTGCTPepBA-3′	−13
3PB	5′ -TPepBGTAAACPepBCATGATGTGCTGCTPepBA-3′	−9
4PB	5′-TPepBGTAPepBAACCPepBATGATGTGCTGCTPepBA-3′	−5
2PK	5′-TPepKGTAAACCATGATGTGCTGCTPepKA-3′	−13
3PK	5′-TPepKGTAAACPepKCATGATGTGCTGCTPepKA-3′	−9
2PC	5′-TPepCGTAAACCATGATGTGCTGCTPepCA-3′	−11
3PC	5′ -TPepCGTAAACCAPepCTGATGTGCTGCTPepCA-3′	−6
2PG	5′-TPepGGTAAACCATGATGTGCTGCTPepGA-3′	−13
3PG	5′ -TPepGGTAAACCAPepGTGATGTGCTGCTPepGA-3′	−9
2PJ	5′-TPepJGTAAACCATGATGTGCTGCTPepJA-3′	−9
3PJ	5′-TPepJGTAAACCAPepJTGATGTGCTGCTPepJA-3′	−3

Abbreviations: ACHN, acetylated N terminus; NH₂, amidated C terminus; PepB, AcHN-Lys-Lys-Lys(N₃)-Lys-Gly-NH₂; PepK, N₃-Gly-Arg-Arg-Arg-Gly-NH₂; PepC, AcHN-Gly-Lys(N₃)-Gly-Arg-Arg-Gly-Arg-Arg-Gly-NH₂; PepG, AcHN-Lys(N₃)-Arg-Arg-Arg-Gly-NH₂; PepJ, AcHN-Lys(N₃)-Lys-Lys-His-His-His-NH₂; TP ODN, triazolylphosphonate oligodeoxynucleotide.

These sequences are anti-miR oligonucleotides (AMOs) to miR-15b (see Figure 7).