Figure 3.

Eupatolide inhibits epithelial-mesenchymal transition through the downregulation of Snail and Slug expression. (A) MDA-MB-231 and (B) MCF-7 cells were treated with 10 µM eupatolide for the indicated times, and the expression of vimentin and E-cadherin were examined by western blotting. (C) MDA-MB-231 and MCF-7 cells were treated at the indicated concentrations of eupatolide for 24 h, and the expression levels of Snail and Slug were examined by western blotting. (D) Levels of Snail and Slug mRNA were examined using reverse transcription-polymerase chain reaction performed in duplicate; no significant differences were observed. EuTL, eupatolide.