Figure 1.

Effect of artesunate on the rate of EOMA cell growth inhibition and the induction of apoptosis in artesunate-treated EOMA cells. (A) Growth inhibition was determined using the MTT assay. Cells were treated with various concentrations of artesunate for the indicated times. The IC₅₀ value of artesunate in EOMA cells was 300 µg/ml. The growth of EOMA cells was significantly inhibited by artesunate in a dose- and time-dependent manner. Results presented are the representative data of at least three independent experiments. (B) Annexin V/PI binding assay analysis of apoptosis in artesunate treated EOMA cells lines. The percentages of early apoptosis (Annexin V+/PI-, LR) are depicted. The results are expressed as mean ± standard deviation from three independent experiments (24 h, P<0.05 vs. 0 h; 48 h, P<0.01 vs. 0 h). (C) Representative images of the respective treatments in the Annexin V/PI binding assay. Results presented are representative data of at least three independent experiments. PI, propidium iodide; FITC, fluorescein isothiocyanate.