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. 2017 Mar 20;16(2):143–151. doi: 10.1002/rmb2.12019

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© 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Apoptosis analysis. The Annexin‐V+/PI– cells show early apoptosis and the Annexin‐V+/PI+ cells show late apoptosis. The average total (early + late) apoptosis rate of the control cells was 12.8% (average early apoptosis, 11.3%; average late apoptosis, 1.5%). The average total apoptosis rate of the DNA‐transfected cells was 72.8% (average early apoptosis, 52.6%; average late apoptosis, 19.7%). There was a significant increase in apoptosis of the DNA‐transfected cells. *P<.0001, compared to the control. The average total apoptosis rate of the small interfering RNA (siRNA)‐transfected cells was 29.9% (average early apoptosis, 27.6%; average late apoptosis, 2.3%). There was a significant increase in apoptosis of the siRNA‐transfected‐cells. **P<.001, compared to the control. FITC , fluorescein isothiocyanate