Figure 1.

Effect of dedidual stimulation on the phenotype of the human eutopic endometrial stromal cells (HEuESCs) and the human ectopic endometrial stromal cells (HEcESCs). Light microscopic images of the decidual transformation of (A,B) the HEuESCs and (C,D) the HEcESCs in vitro. The confluent monolayers were treated with or without 8‐bromo‐cyclic adenosine monophosphate (8‐bromo‐cAMP) (0.5 mmol L⁻¹) and medroxyprogesterone acetate (MPA) (10^–6 mol L⁻¹) for 6 days. The medium was changed on day 3. The expression levels of (E) insulin‐like growth factor‐binding protein 1 (IGFBP1) and (F) prolactin (PRL) microRNA were measured by quantitative real‐time polymerase chain reaction amplification. The confluent HEuESCs or HEcESCs were treated as indicated for 6 days. The medium was changed on day 3. The data are presented as the mean±standard error of the mean. *P<.05 and **P<.01. Scale bar, 200 μm