Figure 5.

Impact of microRNA (miR)‐542‐3p overexpression on the decidual phenotype. A, A quantitative real‐time polymerase chain reaction amplification (qRT‐PCR) analysis of the miR‐542‐3p expression levels in the human eutopic endometrial stromal cells (HEuESCs) and the human ectopic endometrial stromal cells (HEcESCs) that had been treated with or without 8‐bromo‐cyclic adenosine monophosphate (8‐br‐cAMP) and medroxyprogesterone acetate (MPA) for 6 days. The expression of miR542‐3p was calculated as the fold change, relative to the HEuESCs or HEcESCs. B, A qRT‐PCR analysis of the insulin‐like growth factor‐binding protein 1 (IGFBP1) transcript levels in the HEcESCs that were transfected with a miR‐542‐3p mimic or NC mimic and decidualized for 6 days. The expression levels were normalized to glyceraldehyde 3‐phosphate dehydrogenase. The data are presented as the mean±standard error of the mean of three independent experiments. **P<.01