Table 2.
Estimated Concentrations of Redox- and Glutathione-Dependent Enzymes in Yeast
| Estimated concentrationa | |||||
|---|---|---|---|---|---|
| Protein/gene | Essential? | Localization | (100) | (152) | (48) |
| GR/GLR1 | Nob (106, 153, 192, 195, 216) | Cyt, Nu?, MM (215) | 0.44 μM | 1.5 μM | 18 nM |
| Grx1/GRX1 | Nob (72, 73, 151, 168, 192) | Cyt, Nu? | 0.18 μM | 6.5 μM | 27 nM |
| Grx2/GRX2 | Nob (72, 73, 168, 192, 240) | Cyt, MM, IMS (151, 223) | 2.1 μM | 2.3 μM | 3.4 nM |
| Grx3/GRX3 | Nob (194, 231, 240) | Cyt, Nu (188) | 0.65 μM | 0.30 μM | 6.8 nM |
| Grx4/GRX4 | Nob (194, 231, 240) | Cyt, Nu (166) | 0.46 μM | 0.14 μM | — |
| Grx5/GRX5 | Nob (240, 241) | MM (241) | 10 μM | 17 μM | 0.5 μM |
| Grx6/GRX6 | No (132, 184) | ER and cis-Golgi (132, 184) | 3.9 μM | 4.1 μM | 0.19 μM |
| Grx7/GRX7 | No (132, 184) | cis-Golgi (132, 184) | — | 35 μM | 0.38 μM |
| Grx8/GRX8 | No (73) | Cyt (123) | 40 nM | 0.16 μM | — |
| Gto1/GTO1 | No (20) | Per (20) | — | — | — |
| Gto2/GTO2 | Nob (20) | Cyt (20) | 0.12 μM | 37 nM | — |
| Gto3/GTO3 | Nob (20) | Cyt (20) | — | — | — |
| Gtt1/GTT1 | No (20, 47, 52) | ER-associated (47) | — | 0.74 μM | — |
| Gtt2/GTT2 | Nob (20, 47, 52) | ? | — | — | — |
| Ure2/URE2 | Nob (292) | Cyt (74) | 0.49 μM | 71 nM | 14 nM |
| TR1/TRR1 | Nob (274) | Cyt | 20 μM | 4.2 μM | 0.12 μM |
| TR2/TRR2 | Nob (221) | MM (221) | 0.69 μM | 0.94 μM | — |
| Trx1/TRX1 | Nob (72, 274) | Cyt | 0.59 μM | 17 μM | 69 nM |
| Trx2/TRX2 | Nob (72, 274) | MM | 1.2 μM | 7.0 μM | 0.16 μM |
| Trx3/TRX3 | No (221) | MM (221) | 1.7 μM | 25 μM | — |
| GPx1/GPX1 | No (14, 208) | Per (208) | — | 1.3 μM | — |
| GPx2/GPX2 | No (14, 130) | Cyt, Nu? (123) | 0.12 μM | 1.2 μM | 24 nM |
| GPx3/GPX3 | Nob (14, 59, 130) | Cyt (123) | 0.56 μM | 2.3 μM | 9 nM |
| Tsa1/TSA1 | Nob (42, 219, 298) | Cyt (219) | 26 μM | 12 μM | 0.51 μM |
| Tsa2/TSA2 | Nob (219, 298) | Cyt (219) | 0.33 μM | 0.18 μM | — |
| Ahp1/AHP1 | Nob (157, 219, 298) | Cyt (219) | 1.1 μM | 31 μM | 1.3 μM |
| nTPx/DOT5 | No (41, 219, 298) | Nu (219) | 0.76 μM | 1.7 μM | 17 nM |
| mTPx/PRX1 | No (109, 219, 222, 298) | MM (219) | 7.5 μM | 30 μM | 0.18 μM |
| Cta1/CTA1 | Nob (50, 107, 227) | Per, MM (227) | 0.69 μM | — | — |
| Ctt1/CTT1 | Nob (50, 107, 227) | Cyt (123) | 22 nM | 0.13 μM | — |
| Sod1/SOD1 | Nob (25, 285) | Cyt, Nu, IMS (265) | 31 μM | 71 μM | 0.23 μM |
| Glo1/GLO1 | No (128) | Cyt | 0.18 μM | 0.56 μM | — |
| Glo2/GLO2 | No (29) | Cyt (29) | 0.95 μM | 0.53 μM | 26 nM |
| Glo4/GLO4 | No (29) | MM (29) | — | 0.18 μM | — |
| ADH5/ADH5 | No | Cyt (123) | 0.58 μM | 0.71 μM | 23 nM |
The concentration was calculated based on protein abundances from Refs. (48, 100, 152) as summarized in the Saccharomyces Genome Database (www.yeastgenome.org). Molecules per cell were divided by the Avogadro constant and the estimated compartment volume. Averaged cell and organelle volumes are based on Ref. (277): whole cell, 50 fl (μm3); cytosol, 25 fl; nucleus, 4 fl; vacuole, 4fl; mitochondria, 1 fl; endoplasmic reticulum, 0.5 fl; peroxisomes, 0.5 fl; cis-Golgi, 0.2 fl. Compartment volumes were combined for dual localized proteins.
Combined gene deletions resulted in synthetic lethality.
Cyt, cytosol; ER, endoplasmic reticulum; IMS, mitochondrial intermembrane space; Nu, nucleus; MM, mitochondrial matrix; Per, peroxisomes.