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. 2017 Oct 25;10:5137–5149. doi: 10.2147/OTT.S146423

Figure 1.

Figure 1

Upregulation of MEG3 increased the sensitivity of DDP-resistant NSCLC cells to DDP.

Notes: (A) qRT-PCR analysis of MEG3 expression in the tumor tissues of 25 DDP-sensitive and 23 DDP-resistant NSCLC patients. (B) IC50 value was determined by MTT assay after parental (A549, H1299) and DDP-resistant (A549-DDP and H1299-DDP) NSCLC cells were exposed to different concentrations of DDP (0, 2, 4, 8, 16, 32, and 64 μM) for 48 h. (C) qRT-PCR analysis of MEG3 expression in A549, A549-DDP, H1299, and H1299-DDP cells. (D) qRT-PCR analysis of MEG3 expression in MEG3-transfected A549-DDP cells and si-MEG3-introduced H1299-DDP cells. (E) IC50 value was assessed by MTT assay in MEG3-transfected A549-DDP cells and si-MEG3-introduced H1299-DDP cells following treatment with different concentrations of DDP (0, 2, 4, 8, 16, 32, and 64 μM) for 48 h. *P<0.05.

Abbreviations: MTT, 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide; NSCLC, non-small cell lung cancer; qRT-PCR, quantitative real-time polymerase chain reaction; si-NC, nonspecific oligonucleotides.