TABLE 1. Accuracy of fluorescence microscopy and glutaraldehyde (GA) test using a culture reference standard.
| All specimens N = 272 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Methods | Culture | Sensitivity (%) | 95% CI | Specificity (%) | 95% CI | PPV (%) | 95% CI | NPV (%) | 95% CI | |||
| Positive | Negative | |||||||||||
| Fluorescence microscopy | Positive | 56 | 0 | 57.1 | 46.8-67 | 100 | 97.3-100 | 100 | 92-100 | 80.6 | 74.5-85.5 | |
| Negative | 42 | 174 | ||||||||||
| GA test | Positive | 93 | 0 | 94.2 | 87.9-98.1 | 80.5 | 73.6-85.9 | 73.2 | 64.5-80.5 | 96.8 | 91.7-98.7 | |
| Negative | 5 | 174 | ||||||||||
| Respiratory specimens, N = 176 | ||||||||||||
| Fluorescence microscopy | Positive | 42 | 0 | 65.6 | 52.6-76.8 | 100 | 95.9-100 | 100 | 89.6-100 | 83.6 | 76-89.2 | |
| Negative | 22 | 112 | ||||||||||
| GA test | Positive | 62 | 0 | 96.9 | 88.2-99.5 | 82.1 | 73.5-88.5 | 75.6 | 64.7-84.1 | 97.9 | 91.8-99.6 | |
| Negative | 2 | 112 | ||||||||||
| Non respiratory specimens, N = 96 | ||||||||||||
| Fluorescence microscopy | Positive | 14 | 0 | 41.2 | 25.1-59.2 | 100 | 92.7-100 | 100 | 73.2-100 | 75.6 | 64.7-84.1 | |
| Negative | 20 | 62 | ||||||||||
| GA test | Positive | 31 | 0 | 91.2 | 75.2-97.7 | 77.4 | 64.7-86.7 | 68.9 | 53.2-81.4 | 94.1 | 82.8-98.5 | |
| Negative | 3 | 62 | ||||||||||
CI: confidence interval; NPV: negative predictive value; PPV: positive predictive value.