Table 2.
Known miRNAs with significantly differential expression in sugarcane buds following cold treatment
| miRNA | sequence | F0-std | F3-std | R0-std | R3-std | Fold change | |
|---|---|---|---|---|---|---|---|
| F0-F3 | R0-R3 | ||||||
| miR1310 | GAGGCATCGGGGGCGCAACGCC | 8.32 | 29.24 | 15.78 | 30.98 | 1.81** | 0.97 |
| miR1520d | ATCAGAACTGGTACGGACAA | 574.08 | 1917.98 | 1131.16 | 2620.83 | −1.74** | 1.21 |
| miR157a | TTGACAGAAGATAGAGAGCAC | 4.65 | 9.68 | 4.34 | 4.26 | 1.06** | −0.02 |
| miR2199 | TGATAACTTGACGGATCGC | 59.42 | 179.46 | 129.61 | 272.82 | 1.59** | 1.07** |
| miR2916 | GGGGCTCGAAGACGATCAGAT | 46.30 | 198.06 | 128.65 | 255.78 | −2.10** | 0.99 |
| miR5054 | TCCCCACGGACGGCGCCA | 50.00 | 117.65 | 81.84 | 164.77 | 1.23** | 1.00** |
| miR5059 | CGTTCCTGGGCAGCAACACCA | 35.92 | 97.80 | 84.02 | 201.88 | 1.44** | 1.26** |
| miR5072 | GTTCCCCAGCGGAGTCGCCA | 2.16 | 7.70 | 5.34 | 20.97 | 1.82** | 1.97** |
| miR5077 | TTCACGTCGGGTTCACCA | 104.90 | 321.31 | 104.90 | 321.30 | 1.62** | 1.62** |
| miR5152-3p | AGTCCTGCTATACCCACCA | 0.59 | 2.02 | 1.30 | 3.76 | 1.77** | 1.52** |
| miR5575 | TGGATTTTGGAATGTTTTGGT | 0.39 | 1.98 | 3.03 | 2.23 | 2.32** | −0.44 |
| miR5671 | CATGGTGGTGACGGGTGAC | 32.73 | 70.96 | 72.74 | 126.88 | 1.12** | 0.80 |
| miR5813 | ACAGCAGGACGGTGGTCATGGA | 63.41 | 206.30 | 132.57 | 269.48 | 1.70** | 1.02** |
| miR6196 | GGGACGAGAAAGATGGGAGGA | 5.12 | 11.12 | 17.93 | 21.15 | 1.12 | 1.09** |
| miR6300 | GTCGTTGTAGTATAGTGGTG | 101.38 | 217.53 | 216.10 | 284.36 | 1.10** | 0.40 |
| miR6478 | CCGACCTTAGCTCAGTTGGTA | 14.70 | 32.52 | 30.53 | 51.46 | 1.14** | 0.75 |
| miR8155 | CGTAACCTGGCTCCGATACCA | 4.14 | 9.20 | 8.10 | 16.96 | 1.15** | 1.06** |
| miR894 | GTTTCACGTCGGGTTCACCA | 334.04 | 1108.12 | 1040.41 | 2131.64 | 1.72** | 1.03 |
| miR160a | TGCCTGGCTCCCTGTATGCCA | 13.92 | 4.43 | 4.64 | 8.89 | −1.65** | 0.94 |
| miR169b | CAGCCAAGGATGACTTGCCGG | 43.54 | 20.76 | 38.28 | 39.38 | −1.06** | 0.04 |
| miR169e-3p | GGCAGTCTCCTTGGCTAGC | 73.19 | 31.02 | 14.24 | 21.84 | −1.24** | 0.62 |
| miR397-5p | TTGACTGCAGCGTTGATGAGC | 9.82 | 12.34 | 2.30 | 3.64 | 1.12** | 0.66 |
| miR528-5p | TGGAAGGGGCATGCAGAGGAG | 123.62 | 49.19 | 303.86 | 262.15 | −1.32** | −0.21 |
| miR5532 | ATGGAAATTGATGACAAAGGGG | 1.58 | 0.77 | 1.46 | 1.20 | −1.03 * | −0.28 |
| miR1861d | TGCGTCATCAGGCAGGAACTGG | – | – | 3.30 | 15.34 | – | 2.22** |
| miR395o-3p | TGAAGTGTTTGGGTGAACTC | 2.60 | 1.64 | 0.88 | 2.56 | −0.66 | 1.54** |
| miR5221 | AACGAGATGGTGTTTTACTT | – | – | 3.00 | 14.23 | – | 2.24** |
| miR5242 | TATTTAGAACAGGCGATGTCA | 1.26 | 1.49 | 3.68 | 13.24 | 0.24 | 1.84** |
| miR5272f | GAATTGATTTGTGTTGGATAAATT | 3.27 | 2.64 | 0.80 | 1.78 | −0.30 | 1.14** |
| miR394a | TTGGCATTCTGTCCACCTCC | 4.26 | 7.78 | 0.88 | 0.74 | 1.03** | −0.96 |
| miR863-5p | TTATAGTCTTGTGGATCAAAT | – | – | 0.23 | 1.53 | – | 2.73** |
F0 and F3 represent the bud sample from sugarcane cultivar FN39 with 0 h and 3 h cold (4 °C) treatment, respectively, while R0 and R3 represent the similar samples from cultivar ROC22, respectively. ** and * indicate a significant difference at p-value <0.01 and p-value <0.05, respectively. “---” indicates no expression