Table 3.
Novel miRNAs with significant differential expression in sugarcane buds following cold treatment
| miRNA | sequence | F0-std | F3-std | R0-std | R3-std | Fold change | |
|---|---|---|---|---|---|---|---|
| F3-F0 | R3-R0 | ||||||
| novel_mir_107 | AGGGATGGTATGCACTGAAGC | 0.01 | 3.71 | 0.01 | 4.55 | 8.54** | 8.83** |
| novel_mir_117 | AAGAGGAAGAGAGAGAGGGTG | 0.01 | 0.01 | 0.01 | 12.33 | – | 10.27** |
| novel_mir_134 | GAGGCAAGGAGGTGGAATAGAC | 0.01 | 3.90 | – | – | 8.61** | – |
| novel_mir_136 | AAGCAAGTTGGGGTAGGCTAA | 0.01 | 1.30 | – | – | 7.02** | – |
| novel_mir_142 | TGAAACGGTACGGCTGATAAGTT | 0.01 | 1.06 | – | – | 6.73** | – |
| novel_mir_21 | TAAATATATAACATCAGGACC | 1.10 | 0.01 | – | – | −6.79** | – |
| novel_mir_34 | CGTACCACCGTCCGGGACTAA | 1.14 | 0.01 | – | – | −6.84** | – |
| novel_mir_37 | TCCAATGCATACCGTCCCTAA | 3.94 | 0.01 | 5.03 | 0.01 | −8.62** | −8.97** |
| novel_mir_41 | CCGAGAGGTAGGGCCGGTCGAA | 6.55 | 0.01 | 12.83 | 10.34 | −9.35** | – |
| novel_mir_54 | TCCAAATTATAAGACGTTTTG | 1.03 | 0.34 | 1.57 | 0.79 | −1.60** | −1.00 * |
| novel_mir_57 | GGGCTGGTTCGGCTGGTGGAA | 1.10 | 0.01 | – | – | −6.79** | – |
| novel_mir_68 | ATAAGACGTTTTGGCTTTTCT | 3.90 | 0.01 | – | – | −8.61** | – |
| novel_mir_72 | AAGAGGAAGAGAGAGAGGGTGA | 16.09 | 0.01 | 16.17 | 0.01 | −10.65** | −10.66** |
| novel_mir_74 | TCTTGGATTTGCATTGGATGCC | 1.18 | 0.01 | – | – | −6.89** | – |
| novel_mir_79 | CGGCCGACGCGCCGCGGCGGGC | 2.64 | 0.01 | 2.19 | 0.01 | −8.05** | −7.77** |
| novel_mir_80 | TGGTTGTTGGCTGGCCATGGCTG | 5.44 | 5.93 | 6.57 | 0.01 | – | −9.36** |
| novel_mir_81 | GCTAGAGGCAGCAACTGCATA | 9.94 | 0.01 | – | – | −9.96** | – |
| novel_mir_89 | CAATCGTGGACCAACTAGGCT | 4.85 | 0.01 | 3.53 | 4.01 | −8.92** | 0.18 |
| novel_mir_91 | CCTGCGTCGCACGGATTCGT | 1114.45 | 3231.35 | – | – | 1.54** | – |
| novel_mir_97 | GTCGTCGCCGTCGTCGTCGTC | 1.10 | 0.34 | 1.19 | 0.79 | −1.71** | −0.60 |
| novel_mir_1 | GGAATGTTGTCTGGTCGGAGA | 9.31 | 7.37 | 10.48 | 0.01 | −0.34 | −10.03** |
| novel_mir_130 | GGCATGGGAACATGTAGGAAGG | – | – | 0.01 | 2.81 | – | 8.14** |
| novel_mir_171 | TTTGAATAAGACGAGTGGTCA | – | – | 10.33 | 0.01 | – | −10.01** |
| novel_mir_183 | TAAGCTGGCTGATGCTGTTTT | – | – | 1.65 | 0.01 | – | −7.37** |
| novel_mir_216 | TCGAGGCCGAACGGATAAGTC | – | – | 0.01 | 1.53 | – | 7.26** |
| novel_mir_228 | TTTTTGGTGATTGATGACAAC | – | – | 0.01 | 2.90 | – | 8.18** |
| novel_mir_28 | AGGTCATGCTGTAGTTTCATC | – | – | 1.30 | 3.02 | – | 1.21** |
| novel_mir_3 | GGCTGAGCATGCCGCCGTCGAG | 0.75 | 1.49 | 7.18 | 2.40 | 1.00 | −1.58** |
| novel_mir_58 | CGTCGCCGTCGTCGTCGTCGTC | 2.29 | 1.64 | 1.11 | 0.50 | −0.48 | −1.16 * |
| novel_mir_64 | AGGGGTTGTGCGGCGGTGCCC | – | – | 1.42 | 0.01 | – | −7.15** |
| novel_mir_67 | AAAAACTTTGTACTAAAGCATA | – | – | 2.27 | 0.01 | – | −7.82** |
F0 and F3 represent the bud sample from sugarcane cultivar FN39 with 0 h and 3 h cold treatment, respectively; R0 and R3 represent the bud sample from sugarcane cultivar ROC22 with 0 h and 3 h, respectively. ** and * indicate a significant differences at p-value <0.01 and p-value <0.05, respectively. “---” indicates no expression