Table 1.
Summary of temperature and CO2-related effects and their interaction on compounds analysed via 1H–NMR-Spectroscopy and HPLC
| Boreogadus saida | Gadus morhua | ||||||
|---|---|---|---|---|---|---|---|
| Compound | Class | Temperature | CO2 | Interaction | Temperature | CO2 | Interaction |
| Ace | Energy metabolism | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. |
| Lac | Energy metabolism | **,↓ | n.s. | n.s. | n.s. | *↑ | * |
| Suc | Energy metabolism | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. |
| Ala | GABA metabolism | ***,↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| GABA | GABA metabolism | ***,↓ | *,↑ | n.s. | ***,↓ | n.s. | * |
| Gln | GABA metabolism | *,↓ | n.s. | n.s. | *,↑↓ | n.s. | n.s. |
| Glu | GABA metabolism | ***,↓ | n.s. | *** | ***,↓ | n.s. | n.s. |
| Cho | Membrane component | n.s. | n.s. | n.s. | n.s. | n.s. | ** |
| Gpcho | Membrane component | ***,↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| Pcho | Membrane component | **,↑↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| Put | Membrane component | ***,↑↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| AcHis | Osmolyte | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. |
| MI | Osmolyte | ***,↓ | ***,↑ | n.s. | ***,↓ | n.s. | n.s. |
| NAA | Osmolyte | ***,↑ | n.s. | n.s. | ***,↑ | n.s. | n.s. |
| Tau | Osmolyte | **,↑↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| TMAO | Osmolyte | ***,↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| Gly | Other | *,↓ | n.s. | n.s. | ***,↓ | n.s. | n.s. |
| HIAA/5-HT | Other | *,↑ | n.s. | n.s. | *,↑ | n.s. | n.s. |
Compound classes are assigned to match the grouping of each compound as used throughout the discussion of the manuscript
* = p < 0.05, ** = p < 0.01, *** = p < 0.001. ↑ and ↓ indicate either an increase or a decrease of the respective compound with rising temperature or rising CO2. ↑↓ indicates apparent uneven effects. Interactive effects are per definition uneven and were therefore not characterized. A plot for each component is available in the (Additional file 4: Figure S1 and Additional file 5: Figure S2 (NMR) and Additional file 6: Figure S3 (HPLC))
Ace Acetate, AcHis Acetyl-histidine, Ala Alanine, Cho Choline, GABA Gamma-aminobutyric acid, Glu Glutamate, Gln Glutamine, Gpcho Glycerophosphocholine, Gly Glycine, Lac Lactate, MI Myo-inositol, NAA N-acetylaspartate, Pcho Phosphocholine, Put Putrescine, Suc Succinate, Tau Taurine, HIAA 5-Hydroxyindoleacetic acid, 5-HT 5-Hydroxytryptamine (Serotonine)