(A) In contrast to the colons of mice treated with hypotonic 2-PMPA enema, colons of saline treated control mice had extensive hyperplasia and contained malformed stools. H&E stained colon sections at lower (magnification: 4×; scale bar = 200 μm) and higher (magnification: 20×; scale bar = 100 μm) magnification, further revealed local alterations of cellular structure, neutrophil infiltration, and tissue hyperplasia in untreated control mice. (B) Quantitative histopathological scoring (n = 7 mice per group) based on tissue hyperplasia, destruction of cellular architecture, neutrophil infiltration and inflammation also showed a significant reduction in disease with hypotonic 2-PMPA enema treatment compared to untreated mice. *p < 0.05.