Skip to main content
. Author manuscript; available in PMC: 2017 Dec 13.
Published in final edited form as: Cell Rep. 2016 Dec 13;17(11):2955–2965. doi: 10.1016/j.celrep.2016.11.042

Figure 4. Tmem258 Haploinsufficiency Exacerbates DSS Colitis.

Figure 4

(A) Mice were administered 2.5% DSS in drinking water for 7 days. Mortality was monitored daily. *p < 0.05 Gehan-Breslow-Wilcoxon test (survival). N = 5-6 mice per genotype for 2 independent experiments.

(B and C) At day 8, mice were assigned an overall health score (severity), colon lengths were measured, and tissue sections were obtained. Histopathological scoring (HistoPath) was based on severity of inflammation, depth of inflammation, and crypt damage. Epithelial proliferation in response to injury was scored independently. Arrows, inflammation; bracket, epithelial proliferation; dashed line, crypt damage. Data represent mean and SD of histopathological scores. *p < 0.05, Students’ t test. Scale bar, 25 μm.

(D) Colon tissue was obtained at day 8 and transcriptomic profiles were measured by RNA-seq. Differentially expressed inflammatory genes in Tmem258+/- mice are displayed.

See also Figures S3-S5.