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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: Cancer Res. 2013 Dec 23;74(4):1238–1249. doi: 10.1158/0008-5472.CAN-13-1407

Figure 3. Effects of Differentiation on EGFRvIII and Marker Expression.

Figure 3

GBMsp were grown either as a suspension culture or adherent culture in tumor stem media or differentiated by withdrawing EGF and FGF and exposing to two separate differentiating conditions; CNTF, BDNF and retinoic acid (CNTF, BDNF, RA) or 5%FBS + 5% donor horse serum (FBS, DHS). A) Immunoblot analysis with anti-EGFRvIII, anti-CD133, anti-GFAP, and anti β-Actin as control. Four separate cell lines were tested; representative blots of GBMsp-6 are shown. B) Whole cell RNA was utilized in RT-PCR analysis with primers specific for EGFRvIII, CD133, and SOX2 gene products. Each experiment was performed in two different GBM sphere lines. Representative gels of GBMsp-8are shown.