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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: J Occup Environ Med. 2016 Aug;58(8 Suppl 1):S62–S71. doi: 10.1097/JOM.0000000000000742

FIGURE 1.

FIGURE 1

Isolation and detection of miRNAs from serum. Schematic outline of serum miRNA isolation, quality control, and profiling analysis. Briefly, 150 μL of serum from DoDSR samples was used to isolate miRNA using affinity column based chemistry. After isolation, a small portion of sample was used in single miRNA RT-qPCR assays to demonstrate successful miRNA analysis. Following successful single qPCR reactions, samples were profiled using qPCR assays for over 150 miRNAs in duplicate in a 384-well plate. Data were then processed and normalized to determine relative expression levels of each miRNA in the serum samples.