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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: Oncogene. 2010 Sep 27;30(2):234–244. doi: 10.1038/onc.2010.414

Figure 2.

Figure 2

Reduction of endogenous JNK2α in NSCLC cell lines. Retroviral infection of shRNAs targeted to JNK2α in HCC-827 or NCI-H2009 cells (shJNK2α). Uninfected cells and shRNAs targeted to a scrambled sequence (shScrambled) were used as controls. (a) Protein samples were separated using 4–20% SDS–PAGE. (b) Relative expression of JNK isoforms using QRT–PCR analysis for HCC-827-infected cells (top) and NCI-H2009-infected cells (bottom). (c) H2009 cells were serum starved for 24h and then stimulated with 500 mM sorbitol. The protein samples were separated using 4–20% SDS–PAGE and detected with the specified antibodies. **-represents the JNK2 isoform. The arrow indicates the JNK1 isoforms.