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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: J Cell Physiol. 2016 Sep 26;232(3):506–516. doi: 10.1002/jcp.25608

Fig. 2. Inhibitory effects of MnTBAP on bleomycin-induced fibrosis in vivo.

Fig. 2

Mice treated with bleomycin (1 U/kg) with or without MnTBAP (3 mg/kg) or equal volume of saline as control were euthanized at day 28. (A) Lung tissue homogenates and (B) liver tissue homogenates from control and treated mice were analyzed for lipid peroxidation and total ROS levels by the TBARS assay. (C) Representative immunohistochemical micrographs of mice lung tissue stained with H&E. (D) Calculated fibrosis score based on the histopathological assessment of H&E-stained slides. (E) BAL fluid from control and treated mice were analyzed for soluble collagen levels by Sircol® assay. Plots are mean ± S.E.M of five animals per sample group. *P <0.05 versus non-treated control. **P <0.05 versus bleomycin treatment.