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. Author manuscript; available in PMC: 2017 Oct 30.
Published in final edited form as: J Cell Physiol. 2016 Sep 26;232(3):506–516. doi: 10.1002/jcp.25608

Fig. 4. Regulation of canonical Wnt signaling by MnTBAP.

Fig. 4

(A) CRL-1490 cells were treated with the indicated concentrations of bleomycin for either 12 or 24 h, and then probed for canonical Wnt proteins LRP6, Wnt5AB, Dvl2, and β-catenin. (B) CRL-1490 cells were pretreated for 1 h with MnTBAP (200 µM), and then treated with bleomycin (10 mU/ml or 25 mU/ml) for 12 h and probed for LRP6, Wnt5AB, Dvl2, and β-catenin proteins. (C) Mice treated with bleomycin (1 U/kg) with or without MnTBAP (3 mg/kg) or equal volume of saline as control were euthanized at day 28. Lung tissue homogenates obtained from control and treated mice were probed for LRP6, Wnt5AB, Dvl2, and β-catenin proteins. Blots were reprobed with β-actin antibody to confirm equal loading of the samples. Representative blots from three independent experiments (in vitro) or three animals per sample group (lung tissue homogenates) are shown. The immunoblot signals were quantified by densitometry. Values are mean ± S.E.M. (n = 3). *P <0.05 versus non-treated control. **P <0.05 versus bleomycin treatment.