TABLE 1.
MnTBAP regulates bleomycin induced angiogenesis markers
| Relative fold regulation (with respect to untreated control cells) in cells treated with |
|||
|---|---|---|---|
| Angiogenesis markers |
Description | Bleomycin | Bleomycin + MnTBAP |
| CCL2 | Chemokine (C-C motif) ligand 2 | 1.815 | −1.0718 |
| CXCL1 | Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) | 1.3755 | −1 |
| CXCL10 | Chemokine (C-X-C motif) ligand 10 | 1.5801 | −2.2974 |
| CXCL5 | Chemokine (C-X-C motif) ligand 5 | 1.4743 | −1.3195 |
| CXCL6 | Chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2) | 1.815 | 1.3195 |
| EFNB2 | Ephrin-B2 | 1.1975 | −1.3195 |
| F3 | Coagulation factor III (thromboplastin, tissue factor) | 1.4743 | −1.0718 |
| FGF1 | Fibroblast growth factor 1 (acidic) | 1.1173 | −1.0718 |
| FGF2 | Fibroblast growth factor 2 (basic) | 3.1602 | 2.639 |
| FGFR3 | Fibroblast growth factor receptor 3 | 1.1173 | −1 |
| FLT1 | Fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) | 1.3755 | −1.3195 |
| HGF | Hepatocyte growth factor (hepapoietin A; scatter factor) | 1.0425 | −1.1487 |
| HIF1A | Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) | 1.1173 | −1 |
| IL1B | Interleukin 1, beta | 1.815 | 1.0718 |
| IL6 | Interleukin 6 (interferon, beta 2) | 3.387 | 2.2974 |
| CXCL8 | Interleukin 8 | 2.0849 | 1.7411 |
| ITGAV | Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) | 1.0425 | −1 |
| MDK | Midkine (neurite growth-promoting factor 2) | 1.0425 | −2.639 |
| NRP1 | Neuropilin 1 | 1.2834 | −1.2311 |
| SERPINE1 | Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 | 1.1975 | −1.4142 |
| TIMP1 | TIMP metallopeptidase inhibitor 1 | 1.0425 | −1.1487 |
| TIMP3 | TIMP metallopeptidase inhibitor 3 | 1.6935 | 1.4142 |
| VEGFB | Vascular endothelial growth factor B | 1.0425 | −1.0718 |
| VEGFC | Vascular endothelial growth factor C | 1.6935 | 1.5157 |
| B2M | Beta-2-microglobulin | 1.3755 | 1.2311 |
mRNA was extracted from untreated CRL-1490 cells or cells treated with bleomycin (10 mU/ml) or cells pre-treated for 1 h with MnTBAP (100 µM) followed by bleomycin (10 mU/ml) treatment for 24 h and transcribed into cDNA. Abundance of angiogenesis specific genes was analyzed by qRT-PCR. Fold regulation between control and treated cells is shown.