Table 1.

Summary of validation outcomes for midazolam in human plasma

Nominal Conc. (ng/mL)	Day 1 (n=6)			Day 2 (n=6)
Midazolam	(Mean±SD) (ng/mL)	CV (%)	Accuracy (%)	(Mean±SD) (ng/mL)	CV(%)	Accuracy (%)
0.500	0.430±0.034	7.95	86.0	0.504±0.033	16.5	101
2.00	1.97±0.211	10.7	98.6	2.06±0.143	6.96	103
20.0	21.8±0.455	2.09	109	20.8±0.686	3.29	104
200	211±4.82	2.28	106	207±4.69	2.27	104
800	768±24.1	3.13	96.0	745±14.1	1.89	93.2
Nominal Conc. (ng/mL)	Day 3 (n=6)			Inter-day (n=18, 3 days)
Midazolam	(Mean±SD) (ng/mL)	CV (%)	Accuracy (%)	(Mean±SD) (ng/mL)	CV(%)	Accuracy (%)
0.500	0.437±0.033	7.49	87.4	0.457±0.062	13.6	91.4
2.00	2.05±0.200	9.74	103	2.03±0.180	8.89	101
20.0	20.9±0.848	4.06	104	21.1±0.761	3.60	106
200	204±3.97	1.94	102	207±5.12	2.47	104
800	755±14.5	1.92	94.3	756±19.6	2.59	94.5