Table 2.

Summary of validation outcomes for 1-hydroxymidazolam in human plasma

Nominal Conc. (ng/mL)	Day 1 (n=6)			Day 2 (n=6)
1- hydroxymidazolam	(Mean±SD) (ng/mL)	CV (%)	Accuracy (%)	(Mean±SD) (ng/mL)	CV(%)	Accuracy (%)
0.500	0.430±0.057	13.2	85.9	0.485±0.052	10.7	97.0
2.00	2.18±0.156	7.17	109	2.02±0.174	8.65	101
20.0	20.5±0.978	4.77	103	21.4±2.06	9.65	107
200	216±11.7	5.44	108	210±7.28	3.46	106
800	807±32.7	4.06	101	790±15.9	2.01	98.7
Nominal Conc. (ng/mL)	Day 3 (n=6)			Inter-day (n=18, 3 days)
1-hydroxymidazolam	(Mean±SD) (ng/mL)	CV (%)	Accuracy (%)	(Mean±SD) (ng/mL)	CV(%)	Accuracy (%)
0.500	0.575±0.036	6.34	115	0.495±0.083	16.7	99.1
2.00	2.11±0.122	5.77	105	2.10±0.158	7.53	105
20.0	20.0±1.06	5.28	100	20.7±1.47	7.10	103
200	207±13.9	6.73	103	211±11.3	5.34	105
800	789±20.3	2.57	98.6	795±24.2	3.04	99.4