Fig. 4.

Film thickness of the ceramides in the condensed phase measured at 37 mN.m⁻¹ (a). Correlation between film thickness and maximum dipole moment of the condensed phase of ceramides (b). Inset resultant perpendicular dipole of the condensed phase of 10:0 Cer (Δ), 12:0 Cer (▼), 14:0 Cer (O), and 16:0 Cer (●). Results are the average of three independent experiments. c Molecular models generated with Alchemy III software (Tripos Ass.) of 16:0 Cer (I) and the proposed arrangement of 10:0 Cer (II) with the sphingosine chain bending over the N-acyl chain. The scales indicate monolayer thickness measured by BAM, and the dashed line shows the probable location of the interface. Modified from Dupuy et al. (2011)