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. 2017 Aug 23;9(5):649–667. doi: 10.1007/s12551-017-0310-6

Fig. 2.

Fig. 2

Protective effect of Tween 20 (a), Tween 40 (b), Tween 80 and TX-100 (d) against hypotonic hemolysis. Human erythrocytes (Ht = 5–20%) in 5 mM PBS buffer (75 mM NaCl), pH 7.4 were incubated in the presence of detergents for 30 min, at 37 °C prior to monitoring hemoglobin release