Fig. 4.

Studying protein–partner interactions with SRCD spectroscopy. a S100A12 in the presence of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes (black) without ions and incubated with liposomes and calcium (red), zinc (green), or both ions (blue). b SRCD spectra of the peptide Sit1 in aqueous solution (black) and titrated with 0.3 mM (red), 0.6 mM (green), and 1.2 mM oleoyl-CoA (OCoA; blue). c SRCD spectra of MEG-14 (black) and S100A12 (red), the weighted sum of the MEG-14 and S100A9 spectra (green), and the spectrum of the solution containing the MEG-14/S100A9 complex (blue). Error bars indicate 1 SD in the repeat measurements. Adapted from Garcia et al. (2013), Lopes et al. (2014a, b), and Orcia et al. (2017) with permission