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Different effects of osteogenic proteins on the morphology of GUV membrane composed of DOPC (2 mg/mL). a GUVs exhibiting spherical shape, b the AnxA5–membrane interaction with DOPC allowing changes between the internal and external media and subsequent loss of the optical contrast without membrane disruption, c the effect on GUV membrane morphology after TNAP interaction, showing excess area and filament formation. Magnification ×60, scale bars 20 μm
